BU06:Team D

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Contents 1 Meeting Schedule 2 Current Progress 2.1 Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE 3 References 3.1 Techniques 3.2 Protocols

Meeting Schedule

Current Progress

Silent Point Mutation: XbaI at bp 2411 of P. luminescens LuxCDABE

                _/_____       <---XbaI cut
           5' AAT CTA GAT 3'
               N   L   D
              Asn Leu Asp

          Triplets for these A.A.'s
               N    L   D
              AAT  TTA GAT
              AAC  TTG GAC
                   CTG
                   CTT
                   CTC
                   CTA

          Codon Usage Bias! (Frequency: per thousand) 
          Leu Codon     E.coliK12      P.lumin
             CUU           11.0          12.1
             CUC           11.0           9.1
             CUA            3.9           9.0
             CUG           52.8          29.6

CUA in P.lum is 9.0/1000, for a silent point mutation of Leucine, CUU or CUC with frequency of 11.0/1000... and its still Leu!!!!

References

Techniques

Protocols