Recombinational Enahancer: Part BBa J3101

From 2006.igem.org

Recombinational Enhancer: Part BBa_J3101[http://partsregistry.org/Part:BBa_J3101]

These are the oligos that we ordered to make the recombinational enhancer.

• The underlined portion of T1 is an EcoRI sticky end
• The underlined portion of T3 is a PstI sticky end site
• The dark blue portions of the top strand oligoes are the bio brick ends.

5'-->3'

T1: AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATAT

T2: CGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGC

T3: ATTTTACTAGTTGCGGCCGCCTGCA

B1: GGCGGCCGCAACTAGTAAAATGCGGTCACAATTTGCACTAGAA

B2: AGCGCATTACGCTGTAAATCGATATTTTGGTCAATTGTTGACACCCGAATCTAGAAGCGGCCGCG

AATTCGCGGCCGCTTCTAGATTCGGGTGTCAACAATTGACCAAAATATCGATTTACAGCGTAATGCGCTTTCTAGTGCAAATTGTGACCGCATTTTACTAGTTGCGGCCGCCTGCA

    GCGCCGGCGAAGATCTAAGCCCACAGTTGTTAACTGGTTTTATAGCTAAATGTCGCATTACGCGAAAGATCACGTTTAACACTGGCGTAAAATGATCAACGCCGGCGG