BU06:Team C
From 2006.igem.org
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| Team Goal: Add Restriction Sites to Biobrick Ends |
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Contents |
Meeting Schedule
Wednesday July 12th, 3pm Ingalls
- Goal: Present info on how to isolate from plasmid, how to PCR and add ends, and protocol/primer design
Current Progress
- Adding Ends PCR! We will use the AgeI restriction site in the middle where two PCR reactions will overlap and we can stick em together!
- How can we test if the restriction sites have been added successfully?
References
Techniques
- [http://www.openwetware.org/wiki/Designing_primers Designing Primers]
Protocols
- [http://www1.qiagen.com/literature/BenchGuide/pdf/1017778_BenchGuide.pdf Qigaen Bench Guide] - Very good general overview of protocols
- [http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
- [http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
- [http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
- [http://www.uark.edu/ua/henrylab/Links/biochemgen/QIAquick_spin.pdf QIAquick Spin Handbook] - For PCR purification and gel extraction
- [http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]
Tools
- [http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
- [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]
