Brown:Projects

From 2006.igem.org

Bacterial Freeze Tag

Overview

Sender Cell

AHL Sensor

'Freeze Machine'

Unfreeze Mechanism

Magnetotactic Bacteria

Avadin-Biotin Complex

To create magnetosomes covered in Avadin. The basic idea is to create a fusion protein from MagA and Avadin, then isolate the magnetic particles, which will now be coated in avadin. Avadin forms one of the strongest bindings known with the molecule biotin.

Bayer, E. A. and Wilchek, M. (1978). The avidin-biotin complex as a tool in molecular biology. Trends Biochem. Sci. 3, N237-N239.

http://www.weizmann.ac.il/Biological_Chemistry/scientist/Bayer/BayerAvidin.html great review

http://www.avidity.com/apps.html current applications of tagging biotin

http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=nucleotide&val=92791044 Artifical synthesized Gene coding for avidin