Brown:Projects
From 2006.igem.org
|
Home People Research iGEM Proposal Coming to Brown? Contact Projects
Contents |
Bacterial Freeze Tag
Overview
Sender Cell
AHL Sensor
The AHL sensor is TetR promoted and forms the Lux R protien which then forms a complex with AHL. This LuxR and AHL complex then activates the pLUXR promoter. Downstream of the pLUXR promoter is the LACI protein. LacI inhibit the pLAC promoter on the "Freeze Machine".
'Freeze Machine'
A promoter that is regulated by LacI will promote the production of LasI, MotB, and cI. This will subsequently inhibit the production of CFP and LasR. In the presence of LacI, however, MotB, LasI, and cI will not be produced. CFP will therefore be produced along with LasR and LacI. This results in the "freezing" of the cell.
