Aromatic Bacteria : Chiba 2006

From 2006.igem.org

Contents 1 concept 2 members 2.1 schedule 3 How it works 3.1 aroma 3.2 light receptor 3.3 carotenoid 4 agenda

concept

Wow!! By lighting bacteria, a refreshing scent stars filling the room. In the dark, the light sensing E.coli will turn orenge. With the red light on, the color of E.coli fades out, and aroma is produced instead.

members

• daisuke abe
• kensuke nakajima
• mayu ikezumi
• shizuka fukushima
• keiichi suzuki

schedule

            9/7   8   9   10  11  12  13  14  15  16   17  18
Daisuke                                                          
Kensuke           x                              
Mayu         x                                               
Shizuka                                                        
Keiichi                                                           

How it works

aroma

Firstly,we are going to make E.coli that smell good!

We are going to get DNA that codes these products synthases.
[http://en.wikipedia.org/wiki/Carene Carene]
[http://en.wikipedia.org/wiki/Limonene Limonene]
[http://en.wikipedia.org/wiki/Pinene Pinene]
[http://en.wikipedia.org/wiki/Linalool Linalool]
[http://en.wikipedia.org/wiki/Geraniol Geraniol]
[http://en.wikipedia.org/wiki/Farnesene Farnesene]

light receptor

Secondary,We are going to make a light receptor that react different wavelength.

PCB reacts redlight (that is about 660nm). If we change PCB to PΦB and PEB , the absorption wavelength shifts 15nm respectively.

These are a structure of chromophore.

carotenoid

agenda

• 9/5--We solved a problem yesterday. PUC-EBI was very dirty so it caused a lot of problem. After cleaning it, We got a clean band of PUC-EBI（linear）.
• 9/4--We want to make PUC-EBIY. We got EBIY (it's orange color),but couldn't get PUC(vector).A band of PUC-EBI（linear）was very weak ,and we could see several bands. So we discussed what caused this problem.
• 8/29--We discussed about the light sensor bacteria.
• 8/12--Our first experiment using an aroma oil(Sweet Flavor-smells like orange). 10μl of oil was necessary for LB medium(solid and liquid) to smell good, or at least, better.
• 8/4 -- We created parts J29021(generator-3 enzymes that is used to produce a lycopene) and J29022(device-light receptor and LacI).
• 8/4 -- We added EuY(J29030) and carene cyclase(J29036) to the Registry. 4 changes were made to carene cyclase to remove restriction sites of ecoR1 and pst1.