Testing Results

From 2006.igem.org

Overview

We decided to integrate check-points into our construction plan, in order to test intermediary part functionality.
There are three basic modules:

• Module 1: Arabinose inducible pBad/araC promoter (I0500) produces temperature-sensitive LacI (LacI ts), which then, upon dimerization, represses the IPTG inducible LacI+ pL promoter (R0011) resulting in a decrease in GFP (E0240)/mRFP (I13507)
• Module 2: In Module 1, insert DNA sequence coding for 434 cI protein instead of the reporters (GFP/mRFP) and then add Prm+ promoter (I12006), which is repressed by 434 cI and add GFP
• Module 3: Take Module 3 and add mRFP (J04450)

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Completed Tests

Oct 8, 2006

• Module 1 (DH5a): IPTG Induction of mRFP
• Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP

Oct 7, 2006

• Module 1 (DH5a-z1): IPTG Induction of GFP
• Module 1 (DH5a): Arabinose Induction of LacI ts and repression of GFP

Oct 5, 2006

• Module 1 (DH5a-z1): Fluorescnece Images of GFP/mRFP

Sept 21, 2006

• Module 1 (DH5a-z1): IPTG Induction of GFP/mRFP

Sept 16, 2006

• Module 1 (DH5a-z1): IPTG Induction of GFP

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Future Tests

1. Surface response (GFP/mRFP) to varying IPTG and Arabinose
2. Absorbance of Arabinose, and Abs/Emi Spectra of GFP/mRFP

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