JCA121504-Construction of pAC-LuxInv

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PCR 837F/R on pSupDlux (1743 bp, NotI/BamHI) Sub into pAC-TetInv (NotI/BamHI) Product is pAC-LuxInv, bp ------------------------------------------------ ca837F Forward NotI oligo for lux GGTATGCGGCCGCTTAATTTTTAAAGTATGGGCAATC ca837R Reverse BamHI oligo for lux CACTTggatccGTAATGACAGATAATTTTACTC

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