BU06:Team C

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Revision as of 15:50, 6 July 2006 (view source) Mkinney86 (Talk | contribs) ← Older edit		Revision as of 16:10, 6 July 2006 (view source) Mkinney86 (Talk | contribs) Newer edit →
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	=== Techniques ===		=== Techniques ===
	<br><br>		<br><br>
		+	[http://www.openwetware.org/wiki/Designing_primers Designing Primers]
	=== Protocols ===		=== Protocols ===
		+	[http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
		+	[http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
		+	[http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
		+	[http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]
		+
		+	=== Tools ===
		+	[http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
		+	[http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]

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Revision as of 16:10, 6 July 2006

Schedule	Research	Fundraising	Resources	Members	Contact

Team Goal: Add Restriction Sites to Biobrick Ends

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Contents 1 Meeting Schedule 2 Current Progress 3 References 3.1 Techniques 3.2 Protocols 3.3 Tools

Meeting Schedule

Wednesday July 12th, 3pm Ingalls

• Goal: Present info on how to isolate from plasmid, how to PCR and add ends, and protocol/primer design

Current Progress

• Adding Ends PCR! We will use the AgeI restriction site in the middle where two PCR reactions will overlap and we can stick em together!
• How can we test if the restriction sites have been added successfully?

References

Techniques

Designing Primers

Protocols

Agarose Gel Electrophoresis DNA Ligation Miniprep Restriction Digest

Tools

NEB Cutter IDT Oligo Analyzer