BU06:Team C

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Team Goal: Add Restriction Sites to Biobrick Ends

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Contents

Meeting Schedule

Wednesday July 12th, 3pm Ingalls

  • Goal: Present info on how to isolate from plasmid, how to PCR and add ends, and protocol/primer design


Current Progress

  • Adding Ends PCR! We will use the AgeI restriction site in the middle where two PCR reactions will overlap and we can stick em together!
  • How can we test if the restriction sites have been added successfully?

References

Techniques

  • [http://www.openwetware.org/wiki/Designing_primers Designing Primers]

Protocols

  • [http://www1.qiagen.com/literature/BenchGuide/pdf/1017778_BenchGuide.pdf Qigaen Bench Guide] - Very good general overview of protocols
  • [http://www.openwetware.org/wiki/Agarose_gel_electrophoresis Agarose Gel Electrophoresis]
  • [http://www.openwetware.org/wiki/DNA_Ligation DNA Ligation]
  • [http://www.openwetware.org/wiki/Miniprep/Qiagen_kit Miniprep]
  • [http://www.uark.edu/ua/henrylab/Links/biochemgen/QIAquick_spin.pdf QIAquick Spin Handbook] - For PCR purification and gel extraction
  • [http://www.openwetware.org/wiki/Restriction_digest Restriction Digest]

Tools

  • [http://tools.neb.com/NEBcutter2/index.php NEB Cutter]
  • [http://www.idtdna.com/analyzer/Applications/OligoAnalyzer/ IDT Oligo Analyzer]

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