BU06:Team C

From 2006.igem.org

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Team Goal: Add Restriction Sites to Biobrick Ends

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Contents 1 Meeting Schedule 2 Current Progress 3 References 3.1 Techniques 3.2 Protocols 3.3 Tools

Meeting Schedule

Wednesday July 12th, 3pm Ingalls

• Goal: Present info on how to isolate from plasmid, how to PCR and add ends, and protocol/primer design

Current Progress

• Adding Ends PCR! We will use the AgeI restriction site in the middle where two PCR reactions will overlap and we can stick em together!
• How can we test if the restriction sites have been added successfully?

References

Techniques

Designing Primers

Protocols

Agarose Gel Electrophoresis DNA Ligation Miniprep Restriction Digest

Tools

NEB Cutter IDT Oligo Analyzer