Berkeley Work Schedule and Progress
From 2006.igem.org
(Difference between revisions)
(24 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
- | ==Work | + | == Current Work: == |
+ | *''Conjugation testing:''<br> | ||
+ | **oriT - Test plasmid mobility<br> | ||
+ | **TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | ||
+ | **Test mobilization construct<br> | ||
+ | *''Construction:''<br> | ||
+ | **Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
+ | *Lock and Key Strength | ||
+ | **Flourescence assays | ||
+ | *''Other'' | ||
+ | **onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
+ | |||
- | < | + | == Milestones: == |
+ | |||
+ | <i><u>November:</u></i><br> | ||
Measurement data for constructs, construction continues | Measurement data for constructs, construction continues | ||
- | September: Project training continues, implementation begins<br> | + | <i><u>September:</u></i><br> |
+ | Project training continues, implementation begins<br> | ||
- | July-August: Theoretical project design and background research on bacterial conjugation and technique training<br> | + | <i><u>July-August:</u></i><br> |
+ | Theoretical project design and background research on bacterial conjugation and technique training<br> | ||
- | May-June: Synthetic Biology primer and project brainstorming <br> | + | <i><u>May-June:</u></i><br> |
+ | Synthetic Biology primer and project brainstorming <br> |
Latest revision as of 09:03, 14 November 2005
Current Work:
- Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- oriT - Test plasmid mobility
- Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Lock and Key Strength
- Flourescence assays
- Other
- onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming