Berkeley Work Schedule and Progress
From 2006.igem.org
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| - | ==Work | + | == Current Work: == |
| + | *''Conjugation testing:''<br> | ||
| + | **oriT - Test plasmid mobility<br> | ||
| + | **TraJf, TraJr - does promoter induction cause conjugation to initiate?<br> | ||
| + | **Test mobilization construct<br> | ||
| + | *''Construction:''<br> | ||
| + | **Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf | ||
| + | *Lock and Key Strength | ||
| + | **Flourescence assays | ||
| + | *''Other'' | ||
| + | **onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?) | ||
| + | |||
| - | <i><u>November: </ | + | == Milestones: == |
| + | |||
| + | <i><u>November:</u></i><br> | ||
Measurement data for constructs, construction continues | Measurement data for constructs, construction continues | ||
| - | September: Project training continues, implementation begins<br> | + | <i><u>September:</u></i><br> |
| + | Project training continues, implementation begins<br> | ||
| - | July-August: Theoretical project design and background research on bacterial conjugation and technique training<br> | + | <i><u>July-August:</u></i><br> |
| + | Theoretical project design and background research on bacterial conjugation and technique training<br> | ||
| - | May-June: Synthetic Biology primer and project brainstorming <br> | + | <i><u>May-June:</u></i><br> |
| + | Synthetic Biology primer and project brainstorming <br> | ||
Latest revision as of 09:03, 14 November 2005
Current Work:
- Conjugation testing:
- oriT - Test plasmid mobility
- TraJf, TraJr - does promoter induction cause conjugation to initiate?
- Test mobilization construct
- oriT - Test plasmid mobility
- Construction:
- Spo0A - QuikChange (Stratagene kit site directed mutagenesis) out the Eco site and finish mobilizable construct for OriTf
- Lock and Key Strength
- Flourescence assays
- Other
- onRFP - Find out cause for onRFP sporadic behaviour during conjugation (tetR presence?)
Milestones:
November:
Measurement data for constructs, construction continues
September:
Project training continues, implementation begins
July-August:
Theoretical project design and background research on bacterial conjugation and technique training
May-June:
Synthetic Biology primer and project brainstorming
