Biosensor Characterisation
From 2006.igem.org
Experiment 1 was carried out using IPTG responsive E-Coli with LacZ to find out what difference the total solution volume makes to the rate of pH change.
| Experiment: | Control | 1B | 1C | 1D |
| LB (ml): | 50 | 25 | 10 | 5 |
| Culture (ml): | 5 | 3.125 | 1.25 | 0.625 |
| Lactose (ml): | 1.25 | 0.781 | 0.313 | 0.156 |
| Ampicillin (μl): | 10 | 6.25 | 2.5 | 1.25 |
| IPTG (μl): | 10 | 6.25 | 2.5 | 1.25 |
| Total volume (ml): | 56.27 | 28.919 | 11.568 | 5.784 |
The solution with total volume 28.1375 ml (1B) produced the fastest initial rate of pH decrease and reached approximately the same final pH as the control (pH 4.84).
The smallest solution volume changed pH at the slowest rate and after an initial decrease in pH, it then increased to 7.87 overnight.
Therefore, in the optimum order, 1B > Control > 1C > 1D
Experiment 2 was carried out to find out what difference the volume of sterile water added to the solution makes to the rate of pH change.
| Experiment: | Control | 2A | 2B | 2C | |
| LB (ml): | 50 | 50 | 50 | 50 | |
| Culture (ml): | 5 | 5 | 5 | 5 | |
| Ampicillin (μl): | 10 | 10 | 10 | 10 | |
| IPTG (μl): | 10 | 10 | 10 | 10 | |
| Sterile water (ml): | 0 | 50 | 25 | 10 | |
| Total volume (ml): | 55.02 | 105.02 | 80.02 | 65.02 |
