Brown:Summer workshops

From 2006.igem.org

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'''Monday June 5, 2006'''
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[[Brown:iGEM portal 2006 |Home]]<Br>
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[[Calendar:Summer_Workshops|Calendar page]]<br>
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'''Location: Our new lab'''
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<Br>
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<Br>
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'''Monday June 5, 2006''' (10am-12pm)
          
          
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HOMEWORK!!! (this will make my workshop shorter)
HOMEWORK!!! (this will make my workshop shorter)
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*1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid].  Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday).
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*1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid].  Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
*2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
*2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.
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'' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br>
'' Please email Karen to join group. All students must have completed lab safety training to work in the lab. <Br> <Br>
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'''Tuesday June 6, 2006'''
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'''Tuesday June 6, 2006''' (10am-12pm)
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Restriction digest <Br>
Restriction digest <Br>
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'''Led by:''' Azeem and Jason <Br> <Br>
'''Led by:''' Azeem and Jason <Br> <Br>
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'''Wednesday June 7, 2006'''
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'''Thursday June 8, 2006''' (1:00pm - 2:30pm)
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Primer Design <Br>
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Bacteria <Br>
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PCR/Gel <Br>
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A tentative list of topics:<Br>
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'''Led by:''' Azeem and Jason <Br> <Br>
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1. bacterial strains<Br>
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2. how to grow bacteria (solid/liquid culture)
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'''Thursday June 8, 2006'''
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<Br>3. how to avoid contamination
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<Br>4. how to make competent cells
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Bacterial cell culture <Br>
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<Br>5. how to grow plasmids within bacteria
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Bacterial transfection<Br>
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<Br>6. antibiotics resistance
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Making plates <Br>
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<Br>7. blue/white selection
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<Br>8. iGEM specific information <Br>
'''Led by:''' Jamie Gagnon <Br> <Br>
'''Led by:''' Jamie Gagnon <Br> <Br>
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'''Friday June 9, 2006'''
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'''Friday June 9, 2006''' (10am-12pm)
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Mini-prep <Br>
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APE <Br>
'''Led by:''' Azeem and Jason
'''Led by:''' Azeem and Jason

Latest revision as of 16:38, 8 June 2006

Home
Calendar page
Location: Our new lab

Monday June 5, 2006 (10am-12pm)


Lab Safety

  • clothing/protection
  • common sense
  • waste
  • storage

Overview of research basics

  • lab notebook
  • resources
  • why am I doing this experiment?

Making stock solutions

  • pipetting
  • weighing
  • graduated cylinders
  • volumetric flasks
  • % solutions (vol/vol, g/vol)
  • molar vs. normal solutions
  • buffers

HOMEWORK!!! (this will make my workshop shorter)

  • 1. You need to make 3% [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/A2153 BSA] in 10mM [http://www.vwrsp.com/catalog/product/index.cgi?catalog_number=VW0125-3&inE=1&highlight=VW0125-3 Acedic Acid]. Do all the necessary calculations. (If you don't know where to start, don't be discouraged - we'll show you on Monday June 5th).
  • 2. You need to make 2 liters of the following solution (all are liquids except for the salt): 10% glycerol, 0.9% NaCl, 0.01% Tween 20, 0.01% Tween 80. Show calculations.

Led by: Ana (10am-12pm)
Lab safety training with engineering group from 1pm - 5pm Please email Karen to join group. All students must have completed lab safety training to work in the lab.

Tuesday June 6, 2006 (10am-12pm)


Restriction digest
Gel electrophoresis
Led by: Azeem and Jason

Thursday June 8, 2006 (1:00pm - 2:30pm)


Bacteria
A tentative list of topics:
1. bacterial strains
2. how to grow bacteria (solid/liquid culture)
3. how to avoid contamination
4. how to make competent cells
5. how to grow plasmids within bacteria
6. antibiotics resistance
7. blue/white selection
8. iGEM specific information
Led by: Jamie Gagnon


Friday June 9, 2006 (10am-12pm)


APE
Led by: Azeem and Jason

Personal tools
Past/present/future years