Timeline

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==<font color='red'>Week 3: 3rd - 7th July</font>==
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[http://2006.igem.org/Minutes Minutes]
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'''<font color='blue'>Objectives for the arsenic sensor:</font>'''
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==<font color='red'>Week 10: 21<sup>th</sup> - 25<sup>th</sup> August</font>==
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#22nd-23rd: Meeting with Cambridge team
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#Finish presentation
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Assess the effects of the:
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For the lab:
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#Get a working arsenic sensor. Obviously.
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#Have the constructs of arsR and lambda cI complete.
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#Site directed mutagenesis on the urease gene with the aim of making it into a biobrick
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#Possible construction of the hybrid promoter, if primers have arrived.
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==<font color='red'>Week 6:  24<sup>th</sup> - 28<sup>th</sup> July</font>==
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#24th-25th: Meeting with the Cambridge and Imperial teams at Cambridge.
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1) bacterial population density on pH
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==<font color='red'>Week 5: 17<sup>th</sup> - 21<sup>st</sup> July</font>==
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#Cambridge presentation meeting thursday.  The output from this will be a good layout for the presentation, which can be improved on friday.
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#PCR primers for Urease part ordered
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#Website host adress (www.macteria.co.uk) acquired
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#Batch of 50 badges made (so we can take them to cambridge)
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#"Turtle" characterised (if it arrives)
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#Investigate available growth mediums in preparation for further experimentation next week
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#Minipreps, digests and gels for cells transformed with (hopefully) biobricked arsenic and lacZ parts.
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#Joining of arsenic and lacZ parts, with a terminator, and transforming into E. coli
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2) growth phase on pH change
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==<font color='red'>Week 4: 10<sup>th</sup> - 14<sup>th</sup> July</font>==
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#Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder
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#Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels
 +
#Devising new pH experiments to calibrate the sensor
 +
#Badge making, postcard making, locating possible people to send them to
 +
#Organising Cambridge trip
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#Initial modelling of the biosensor, finding out all the reactions involved and turning them  into equations
 +
#Set up new website
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3) concentration of lactose on pH
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==<font color='red'>Week 3: 3<sup>rd</sup> - 7<sup>th</sup> July</font>==
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4)  time on pH
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==='''<font color='blue'>Objectives for the arsenic sensor:</font>'''===
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All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli. 
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Assess the effects of the:
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'''Objectives for the 3d structure builder:'''
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:1)  bacterial population density on pH
 +
 
 +
:2)  growth phase on pH change
 +
 
 +
:3)  concentration of lactose on pH
 +
 
 +
:4)  time on pH
 +
 
 +
 
 +
All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli.
 +
 
 +
Due to problems in the lab, the above objectives are revised to next week.  These problems are illustrated in the [http://2006.igem.org/Lab_Work Lab work section].  Objectives for this week are now to get the first PCR primers made to assemble our first biobricks.  Tests for the production of lactic acid on L-agar plates using the LacZ plasmids should be started tomorrow afternoon after the lecture from Dr Elfick.
 +
 
 +
==='''<font color='blue'>Objectives for the 3d structure builder:</font>'''===
1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate
1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate
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==<font color='red'>Week 2: 26th - 30th June</font>==
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==<font color='red'>Week 2: 26<sup>th</sup> - 30<sup>th</sup> June</font>==
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More Brainstorming  
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More Brainstorming
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==<font color='red'>Week 1: 19th - 23rd June</font>==
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==<font color='red'>Week 1: 19<sup>th</sup> - 23<sup>rd</sup> June</font>==
Brainstorming
Brainstorming
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[http://2006.igem.org/University_of_Edinburgh_2006 Main page]
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__NOTOC__

Latest revision as of 09:33, 25 October 2006

Minutes

Week 10: 21th - 25th August

  1. 22nd-23rd: Meeting with Cambridge team
  2. Finish presentation

For the lab:

  1. Get a working arsenic sensor. Obviously.
  2. Have the constructs of arsR and lambda cI complete.
  3. Site directed mutagenesis on the urease gene with the aim of making it into a biobrick
  4. Possible construction of the hybrid promoter, if primers have arrived.

Week 6: 24th - 28th July

  1. 24th-25th: Meeting with the Cambridge and Imperial teams at Cambridge.

Week 5: 17th - 21st July

  1. Cambridge presentation meeting thursday. The output from this will be a good layout for the presentation, which can be improved on friday.
  2. PCR primers for Urease part ordered
  3. Website host adress (www.macteria.co.uk) acquired
  4. Batch of 50 badges made (so we can take them to cambridge)
  5. "Turtle" characterised (if it arrives)
  6. Investigate available growth mediums in preparation for further experimentation next week
  7. Minipreps, digests and gels for cells transformed with (hopefully) biobricked arsenic and lacZ parts.
  8. Joining of arsenic and lacZ parts, with a terminator, and transforming into E. coli

Week 4: 10th - 14th July

  1. Locate parts in the registry for the modelling of the arsenic biosensor and the 3D structure builder
  2. Continuing in the lab with putting the arsR and new lacZ genes into biobrick constructs and trying to solve issues with isolating DNA fragments from gels
  3. Devising new pH experiments to calibrate the sensor
  4. Badge making, postcard making, locating possible people to send them to
  5. Organising Cambridge trip
  6. Initial modelling of the biosensor, finding out all the reactions involved and turning them into equations
  7. Set up new website

Week 3: 3rd - 7th July

Objectives for the arsenic sensor:

Assess the effects of the:


1) bacterial population density on pH
2) growth phase on pH change
3) concentration of lactose on pH
4) time on pH


All effects are to be measured on the pH of the liquid cultures with the Lacz deficient strain of E-coli.

Due to problems in the lab, the above objectives are revised to next week. These problems are illustrated in the Lab work section. Objectives for this week are now to get the first PCR primers made to assemble our first biobricks. Tests for the production of lactic acid on L-agar plates using the LacZ plasmids should be started tomorrow afternoon after the lecture from Dr Elfick.

Objectives for the 3d structure builder:

1) Determine whether to use the Urease or Phosphatase enzyme to precipitate a solid substrate

Week 2: 26th - 30th June

More Brainstorming

Week 1: 19th - 23rd June

Brainstorming


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