BU06:Team A

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| <font face="century gothic">'''Mac Cowell'''
| <font face="century gothic">'''Mac Cowell'''
| <font face="century gothic">[mailto:macowell@bu.edu macowell@bu.edu]     
| <font face="century gothic">[mailto:macowell@bu.edu macowell@bu.edu]     
-
| <font face="century gothic"> aim: cxmac
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| <font face="century gothic"> aim: cxmac2
|-
|-
| <font face="century gothic">'''Christine Woods'''
| <font face="century gothic">'''Christine Woods'''
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== Meeting Schedule ==
== Meeting Schedule ==
 +
Next meeting:
== Current Progress ==
== Current Progress ==
===Photosystem parts===
===Photosystem parts===
-
''Light Sensing Inhibitor:'' [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (21A iGEM2006 DNA-2), [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (21C iGEM2006 DNA-2), [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Must be used in E.coli deficient in natural EnvZ.), [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (15P iGEM2006 DNA-1)
+
''Light Sensing Inhibitor:''  
 +
* [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A plate: iGEM2006 DNA-2)  
 +
* [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C plate: iGEM2006 DNA-2)
 +
* [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
 +
* [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P plate: iGEM2006 DNA-1)
 +
 
 +
'''[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=BU BU's temporary parts]'''
===Assembly of parts===
===Assembly of parts===
-
Here are [[Biobrick delivery|instructions]] for transforming DNA into cells and creating our own stock of DNA from the 2006 iGEM plates as well as figuring out where your part is on the plates.  <br>
+
* [[Biobrick delivery]] - overview of the the 2006 iGEM DNA distribution plates.
-
Note: Any DNA in the DNA 1 plate is Ampicillin resistant but the DNA 2 plate contains either Ampicillin, Ampicillin-Kanamycin, and Kanamycin resistant DNA...we're still figuring out how to determine each part's resistance.
+
 +
* [http://partsregistry.org/Help:How_to_make_agar Color-codes] that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance.  Biobricks stored in Plate 1 are all Ampicilin resistant.  Those in Plate 2 have a color-code).
 +
 +
* [http://openwetware.org/wiki/BioBricks_construction_tutorial biobrick construction tutoral] and related protocols.
 +
** [http://openwetware.org/wiki/Silver:_BB_Strategy Summary] of biobrick construction from the Silver lab.
 +
** [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A assembly] is an interesting alternative that avoids the gel purification step.
==Questions==
==Questions==
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* What strain of e. coli should we use?  How do we make those cells competent?   
* What strain of e. coli should we use?  How do we make those cells competent?   
-
* How do we know which antibiotic Plate 2 DNA is resistant to?   
+
* How do we find out which antibiotic a particular DNA on Plate 2 is resistant to?  The [[Biobrick delivery|Biobrick Delivery instructions]] say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
 +
** The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.
 +
 
 +
* How do we obtain part I15010?
 +
**  Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
-
* How do we obtain part i15010?  How do we obtain EnvZ- e. coli?   
+
* How do we obtain EnvZ- e. coli?
 +
**  Ask frank - he seems to know.  
* Who knows what we can buy now and what general lab supplies are available for our use?
* Who knows what we can buy now and what general lab supplies are available for our use?
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== References ==
== References ==
 +
* [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW.
* The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
* The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
* The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
* The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
 +
* [http://openwetware.org/wiki/Protocols Protocols]
 +
* [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
 +
* Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
 +
* [http://www.hamline.edu/depts/biology/courses/biocon2/luxback.html Background] on the lux operon
 +
* [http://encyclopedia.laborlawtalk.com/bioluminescence Bioluminescence. Interesting.]

Latest revision as of 01:31, 31 July 2006

BU06.jpg
Schedule Research Fundraising Resources Members Contact
Team Goal: Photography System Assembly and Testing


back to the BU iGEM main page

Contents

Members

Mac Cowell macowell@bu.edu aim: cxmac2
Christine Woods cwoods@bu.edu aim: xoptopinope
Nancy Mendonca Davis nancymd@bu.edu
Nadav Ivzan nivzan@bu.edu aim:kingos82 msn:kingos19@hotmail.com

Meeting Schedule

Next meeting:

Current Progress

Photosystem parts

Light Sensing Inhibitor:

  • [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A plate: iGEM2006 DNA-2)
  • [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C plate: iGEM2006 DNA-2)
  • [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
  • [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P plate: iGEM2006 DNA-1)

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=BU BU's temporary parts]

Assembly of parts

  • [http://partsregistry.org/Help:How_to_make_agar Color-codes] that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance. Biobricks stored in Plate 1 are all Ampicilin resistant. Those in Plate 2 have a color-code).
  • [http://openwetware.org/wiki/BioBricks_construction_tutorial biobrick construction tutoral] and related protocols.
    • [http://openwetware.org/wiki/Silver:_BB_Strategy Summary] of biobrick construction from the Silver lab.
    • [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A assembly] is an interesting alternative that avoids the gel purification step.

Questions

  • What strain of e. coli should we use? How do we make those cells competent?
  • How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
    • The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.
  • How do we obtain part I15010?
    • Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
  • How do we obtain EnvZ- e. coli?
    • Ask frank - he seems to know.
  • Who knows what we can buy now and what general lab supplies are available for our use?

Protocols

References

  • [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW.
  • The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
  • The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
  • [http://openwetware.org/wiki/Protocols Protocols]
  • [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
  • Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
  • [http://www.hamline.edu/depts/biology/courses/biocon2/luxback.html Background] on the lux operon
  • [http://encyclopedia.laborlawtalk.com/bioluminescence Bioluminescence. Interesting.]

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