BU06:Team A
From 2006.igem.org
(Difference between revisions)
m (→References) |
m (→References) |
| (4 intermediate revisions not shown) | |
Latest revision as of 01:31, 31 July 2006
|
| Schedule | Research | Fundraising | Resources | Members | Contact |
|---|
| Team Goal: Photography System Assembly and Testing |
back to the BU iGEM main page
Contents |
Members
| Mac Cowell | macowell@bu.edu | aim: cxmac2 | |
| Christine Woods | cwoods@bu.edu | aim: xoptopinope | |
| Nancy Mendonca Davis | nancymd@bu.edu | ||
| Nadav Ivzan | nivzan@bu.edu | aim:kingos82 msn:kingos19@hotmail.com |
Meeting Schedule
Next meeting:
Current Progress
Photosystem parts
Light Sensing Inhibitor:
- [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A plate: iGEM2006 DNA-2)
- [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C plate: iGEM2006 DNA-2)
- [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
- [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P plate: iGEM2006 DNA-1)
[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=BU BU's temporary parts]
Assembly of parts
- Biobrick delivery - overview of the the 2006 iGEM DNA distribution plates.
- [http://partsregistry.org/Help:How_to_make_agar Color-codes] that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance. Biobricks stored in Plate 1 are all Ampicilin resistant. Those in Plate 2 have a color-code).
- [http://openwetware.org/wiki/BioBricks_construction_tutorial biobrick construction tutoral] and related protocols.
- [http://openwetware.org/wiki/Silver:_BB_Strategy Summary] of biobrick construction from the Silver lab.
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A assembly] is an interesting alternative that avoids the gel purification step.
Questions
- What strain of e. coli should we use? How do we make those cells competent?
- How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
- The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.
- How do we obtain part I15010?
- Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
- How do we obtain EnvZ- e. coli?
- Ask frank - he seems to know.
- Who knows what we can buy now and what general lab supplies are available for our use?
Protocols
References
- [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW.
- The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
- The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
- [http://openwetware.org/wiki/Protocols Protocols]
- [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
- Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
- [http://www.hamline.edu/depts/biology/courses/biocon2/luxback.html Background] on the lux operon
- [http://encyclopedia.laborlawtalk.com/bioluminescence Bioluminescence. Interesting.]
