BU06:Team A
From 2006.igem.org
(→References) |
(→Members: formatting's still off) |
||
| Line 8: | Line 8: | ||
[[Category: Boston University 2006| Team A]] | [[Category: Boston University 2006| Team A]] | ||
== Members == | == Members == | ||
| - | + | {| width="400" | |
| + | |- style="background:#E1E1E1;" | ||
| + | | <font face="century gothic">'''Mac Cowell''' | ||
| + | | <font face="century gothic">[mailto:macowell@bu.edu macowell@bu.edu] | ||
| + | | aim: cxmac | ||
| + | |- | ||
| + | | <font face="century gothic">'''Christene Woods''' | ||
| + | | <font face="century gothic">[mailto:cwoods@bu.edu cwoods@bu.edu] | ||
| + | | aim: xoptopinope | ||
| + | | | ||
| + | |- style="background:#E1E1E1;" | ||
| + | | <font face="century gothic">'''Nancy Mendonca Davis''' | ||
| + | | <font face="century gothic">[mailto:nancymd@bu.edu nancymd@bu.edu] | ||
| + | |- | ||
| + | | <font face="century gothic">'''Nadav Ivzan''' | ||
| + | | <font face="century gothic">[mailto:nivzan@bu.edu nivzan@bu.edu] | ||
| + | | aim:kingos82 msn:kingos19@hotmail.com | ||
| + | |} | ||
== Meeting Schedule == | == Meeting Schedule == | ||
Revision as of 21:32, 10 July 2006
| Team Goal: Photography System Assembly and Testing |
back to the BU iGEM main page
Contents |
Members
| Mac Cowell | macowell@bu.edu | aim: cxmac | |
| Christene Woods | cwoods@bu.edu | aim: xoptopinope | |
| Nancy Mendonca Davis | nancymd@bu.edu | ||
| Nadav Ivzan | nivzan@bu.edu | aim:kingos82 msn:kingos19@hotmail.com |
Meeting Schedule
Current Progress
Making E. coli Glow
Preliminary Design: -Light Sensing Inhibitor- -> -Light emission device-
Light Sensing Inihibitor: [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
Light Emission Device: (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. Lux Operon
Point Mutation: Lamda Red - Datsenko, Wanner... or by PCR: look for protocol on OpenWetWare\
Codon Usage Bias Database: http://www.kazusa.or.jp/codon/
Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)
It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].
Part: BBa_F1610 codes for LuxI, should we need it...
References
- The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
Techniques
=== Protocols ===
