BU06:Team A

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== Current Progress ==
== Current Progress ==
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===Making ''E. coli'' Glow===
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===Photosystem parts===
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'''Preliminary Design: ''-Light Sensing Inhibitor- -> -Light emission device-'' '''
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''Light Sensing Inihibitor:'' [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
''Light Sensing Inihibitor:'' [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]
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''Light Emission Device:'' (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. [[Lux_operon| Lux Operon]]
 
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Point Mutation: Lamda Red - Datsenko, Wanner... or by PCR: look for protocol on OpenWetWare\
 
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Codon Usage Bias Database: http://www.kazusa.or.jp/codon/
 
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Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)
 
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It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].
 
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Part: BBa_F1610 codes for LuxI, should we need it...
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===Assembly of parts===

Revision as of 21:38, 10 July 2006

BU06.jpg
Schedule Research Fundraising Resources Members Contact
Team Goal: Photography System Assembly and Testing


back to the BU iGEM main page

Contents

Members

Mac Cowell macowell@bu.edu aim: cxmac
Christene Woods cwoods@bu.edu aim: xoptopinope
Nancy Mendonca Davis nancymd@bu.edu
Nadav Ivzan nivzan@bu.edu aim:kingos82 msn:kingos19@hotmail.com

Meeting Schedule

Current Progress

Photosystem parts

Light Sensing Inihibitor: [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]


Assembly of parts

References

  • The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.


Techniques



=== Protocols ===

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