BU06:Team A

From 2006.igem.org

Team Goal: Photography System Assembly and Testing

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Contents 1 Members 2 Meeting Schedule 3 Current Progress 3.1 Making E. coli Glow 4 References 4.1 Techniques 4.2 Protocols

Members

Meeting Schedule

Current Progress

Making E. coli Glow

Preliminary Design: -Light Sensing Inhibitor- -> -Light emission device-

Light Sensing Inihibitor: [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]

Light Emission Device: (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. Lux Operon

Point Mutation: Lamda Red - Datsenko, Wanner... or by PCR: look for protocol on OpenWetWare\

Codon Usage Bias Database: http://www.kazusa.or.jp/codon/

Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)

It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].

Part: BBa_F1610 codes for LuxI, should we need it...

References

• The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.

Techniques

Protocols