BU06:Team A

From 2006.igem.org

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Team Goal: Photography System Assembly and Testing

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Contents 1 Members 2 Meeting Schedule 3 Current Progress 3.1 Photosystem parts 3.2 Assembly of parts 4 Questions 5 Protocols 6 References

Members

Mac Cowell	macowell@bu.edu	aim: cxmac2
Christine Woods	cwoods@bu.edu	aim: xoptopinope
Nancy Mendonca Davis	nancymd@bu.edu
Nadav Ivzan	nivzan@bu.edu	aim:kingos82 msn:kingos19@hotmail.com

Meeting Schedule

Next meeting:

Current Progress

Photosystem parts

Light Sensing Inhibitor:

• [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A plate: iGEM2006 DNA-2)
• [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C plate: iGEM2006 DNA-2)
• [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ. Also, not in 2006 iGEM dna distribution)
• [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P plate: iGEM2006 DNA-1)

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2006&group=BU BU's temporary parts]

Assembly of parts

• Biobrick delivery - overview of the the 2006 iGEM DNA distribution plates.

• [http://partsregistry.org/Help:How_to_make_agar Color-codes] that indicate the corresponding biobrick's antibiotic resistance. (Each brick has a certain antibiotic resistance. Biobricks stored in Plate 1 are all Ampicilin resistant. Those in Plate 2 have a color-code).

• [http://openwetware.org/wiki/BioBricks_construction_tutorial biobrick construction tutoral] and related protocols.
  • [http://openwetware.org/wiki/Silver:_BB_Strategy Summary] of biobrick construction from the Silver lab.
  • [http://openwetware.org/wiki/Synthetic_Biology:BioBricks/3A_assembly 3A assembly] is an interesting alternative that avoids the gel purification step.

Questions

• What strain of e. coli should we use? How do we make those cells competent?

• How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
  • The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.

• How do we obtain part I15010?
  • Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)

• How do we obtain EnvZ- e. coli?
  • Ask frank - he seems to know.

• Who knows what we can buy now and what general lab supplies are available for our use?

Protocols

References

• [http://openwetware.org/wiki/Synthetic_Biology:BioBricks Synthetic_Biology:BioBricks] TONS of overlooked info & protocols on OWW.
• The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
• The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
• [http://openwetware.org/wiki/Protocols Protocols]
• [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
• Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
• [http://www.hamline.edu/depts/biology/courses/biocon2/luxback.html Background] on the lux operon
• [http://encyclopedia.laborlawtalk.com/bioluminescence Bioluminescence. Interesting.]