Aromatic Bacteria : Chiba 2006

From 2006.igem.org

Contents 1 concept 2 members 2.1 schedule 3 How it works 3.1 aroma 3.2 light receptor 3.3 carotenoid 4 papers 5 news

concept

Wow!! By lighting bacteria, a refreshing scent stars filling the room. In the dark, the light sensing E.coli will turn orenge. With the red light on, the color of E.coli fades out, and aroma is produced instead.

members

• daisuke abe
• kensuke nakajima
• mayu ikezumi
• shizuka fukushima
• keiichi suzuki

schedule

            9/21   22   23   24  25  26  27  28  29  30
Daisuke      x     x                                               
Kensuke                              x     
Mayu                               
Shizuka                 x    x   x   x                          
Keiichi                                                           

How it works

aroma

Firstly,we are going to make E.coli that smell good!

We are going to get gene that codes these products synthases.

[http://en.wikipedia.org/wiki/Carene Carene]
[http://en.wikipedia.org/wiki/Limonene Limonene]
[http://en.wikipedia.org/wiki/Pinene Pinene]
[http://en.wikipedia.org/wiki/Linalool Linalool]
[http://en.wikipedia.org/wiki/Geraniol Geraniol]
[http://en.wikipedia.org/wiki/Farnesene Farnesene]

light receptor

Secondary,We are going to make a light receptor that react different wavelength.

PCB reacts redlight (that is about 660nm). If we change PCB to PΦB and PEB , the absorption wavelength shifts 15nm respectively.

These are a structure of chromophore.

carotenoid

papers

Aroma etc…

[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15236249 Yasuo Yoshikuni(2004)-Mono and diterpene production in Escherichia coli.]

[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=15577217&dopt=Abstract Gisho Honda(2004)cDNA isolation and functional expression of myrcene synthase from Perilla frutescens.]

Light receptor etc…

[http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16458890&dopt=Abstract Takayuki Kohchi(2006)-Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli.]

[http://www.pnas.org/cgi/content/full/101/50/17334?ck=nck J.Clark Lagarias(2004)-Harnessing phytochrome's glowing potential]

carotenoid etc…

news

• 9/23--We succesed yesterday's experiment. The smell of geraniol that is produced E.coli was a kind of citrus.

• 9/22--We tested efficency of E.coli that makes geraniol.

• 9/21--We are trying to make E.coli that makes geraniol.

• 9/12--We got a gene that codes geraniol synthase.

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