BU06:Team A

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[[Category: Boston University 2006| Team A]]
[[Category: Boston University 2006| Team A]]
== Members ==
== Members ==
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{| width="400"
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|- style="background:#E1E1E1;"
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| <font face="century gothic">'''Mac Cowell'''
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| <font face="century gothic">[mailto:macowell@bu.edu macowell@bu.edu]   
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| aim: cxmac
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|-
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| <font face="century gothic">'''Christene Woods'''
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| <font face="century gothic">[mailto:cwoods@bu.edu cwoods@bu.edu]
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| aim: xoptopinope
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|
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|- style="background:#E1E1E1;"
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| <font face="century gothic">'''Nancy Mendonca Davis'''
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| <font face="century gothic">[mailto:nancymd@bu.edu nancymd@bu.edu]
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|-
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| <font face="century gothic">'''Nadav Ivzan'''
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| <font face="century gothic">[mailto:nivzan@bu.edu nivzan@bu.edu]
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| aim:kingos82 msn:kingos19@hotmail.com
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|}
== Meeting Schedule ==
== Meeting Schedule ==

Revision as of 21:32, 10 July 2006

Team Goal: Photography System Assembly and Testing


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Contents

Members

Mac Cowell macowell@bu.edu aim: cxmac
Christene Woods cwoods@bu.edu aim: xoptopinope
Nancy Mendonca Davis nancymd@bu.edu
Nadav Ivzan nivzan@bu.edu aim:kingos82 msn:kingos19@hotmail.com

Meeting Schedule

Current Progress

Making E. coli Glow

Preliminary Design: -Light Sensing Inhibitor- -> -Light emission device-

Light Sensing Inihibitor: [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] , [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] , [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] , [http://partsregistry.org/Part:BBa_R0082 BBa_R0082]

Light Emission Device: (from lux [http://en.wikipedia.org/wiki/Operon operon]) THIS IS WHAT WE NEED TO DO. Lux Operon

Point Mutation: Lamda Red - Datsenko, Wanner... or by PCR: look for protocol on OpenWetWare\

Codon Usage Bias Database: http://www.kazusa.or.jp/codon/

Light Sensor Parts: http://partsregistry.org/Featured_Parts:Light_Sensor and paper : http://www.nature.com/nature/journal/v438/n7067/full/nature04405.html (this paper simply explains how the device works!)

It seems to repress gene expression by having red light inhibit phosphorylation which would activate a promoter. We would replace the LacZ protein coding region with our lux [http://en.wikipedia.org/wiki/Operon operon].

Part: BBa_F1610 codes for LuxI, should we need it...


References

  • The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.


Techniques



=== Protocols ===

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