Tokyo Alliance: Conclusion

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===Data===
===Data===
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In our assay, we measured fluorescence resonance of GFP of E.coli cultured in liquid medium with or without chemicals.
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In our assay, we can measure fluorescence resonance of GFP of E.coli cultured in liquid medium with or without chemicals.
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[[Image:tokyo_data_60.jpg|400px]]
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By addition of a inducer, GFP fluorescence will increase.
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We show GFP expression regulated by yes gate containing lacI binding sites.
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In this graph, X-axis is induction time.  Y-axis is GFP fluorescence.
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This white arrow indicates GFP expression of E.coli cultured without IPTG. By addition of IPTG, we indicate it by red arrow, GFP fluorescence increased 1.5times.
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[[Image:tokyo_data_61.jpg|400px]]
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This is the construct of FadR. By addition of oleate, GFP expression increased 1.5 times.
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[[Image:tokyo_data_62.jpg|400px]]
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This is the construct of AraC.
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By addition of arabinose, GFP expression increased 3 times.
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[[Image:tokyo_data_63.jpg|400px]]
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This is the construct of EmrR.
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By addition of salicilate, GFP expression increased 1.5times.
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[[Image:tokyo_data_64.jpg|400px]]
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This is the construction of LuxR. 
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By addition of AHL, GFPexpression increased 6times.
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==Conclusion==
==Conclusion==

Revision as of 08:33, 7 November 2006

Top : Introduction : Significance : Design/Method : Results/Conclusion : About Tokyo Alliance

Contents

Results

Candidates of Inducible Regulators

We searched several number of, actually at least 7, regulators inducible by different chemicals. This list is the candidates of such a regulator.

Candidates of Inducible Regulators


Construction Family Tree

Using these regulatory sequences, we constructed these iGEM parts. There are 22 different logic gates actually made. Some of them are ANDgates.

Construction Family Tree (rev.1022)

We constructed YES gates and AND gates shown above accorinding to our Procedure.

Construction Index


  • B pSB Lacq prom. #4 J54202
    • B-1 RbsR J54212
    • B-2 FadR J54222
      • B-21 FadR tandem
    • B-3 TetR J54232
    • B-4 LVA
    • B-5 LacI (Library) J54251
      • B-51 LVA
      • B-52 LacI tandem J54252
        • B-52-a1 LacI tandem-TetR


  • C pSB LacI prom. #1


  • D pSB TyrS prom.
    • D-1 TyrS


  • E natural LacI prom.

Tokyo Alliance iGEM registry list

Data

In our assay, we can measure fluorescence resonance of GFP of E.coli cultured in liquid medium with or without chemicals.

By addition of a inducer, GFP fluorescence will increase.

Conclusion

  • Conclusion

We can say we expanded the number of regulator genes we can use to build logic gates and through this project we made simple constructing method.

  • Future Work

It must be possible to construct functioning AND gates with our systematic construction strategy. And with same method, it is possible to make ANDAND gates, also. In order to make Noughts and Crosses game, we need 11 AND gates and 10 ANDAND gates.

Not tested yet, we already have constructed 7AND gates. It may not be so hard to complete the set of logic gates and combine them into Noughts and Crosses.

We recently change transition diagram and found that the number of inputs can be reduced into just 6. So the implementation of SYANAC will be little bit easier.

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