Tokyo Alliance: Conclusion
Candidates of Inducible Regulators
We searched several number of, actually at least 7, regulators inducible by different chemicals. This list is the candidates of such a regulator.
Construction Family Tree
Using these regulatory sequences, we constructed these iGEM parts. There are 22 different logic gates actually made. Some of them are ANDgates.
We constructed YES gates and AND gates shown above accorinding to our Procedure.
- A pSB#4 "pSBΔP" J54103
- B pSB Lacq prom. #4 J54202
- C pSB LacI prom. #1
- D pSB TyrS prom.
- D-1 TyrS
- E natural LacI prom.
In our assay, we can measure fluorescence resonance of GFP of E.coli cultured in liquid medium with or without chemicals.
By addition of a inducer, GFP fluorescence will increase.
We can say we will expand the number of regulator genes we can use to build logic gates and through this project we made simple constructing method.
- Future Work
It must be possible to construct functioning AND gates with our systematic construction strategy. And with same method, it is possible to make ANDAND gates, also. In order to make Noughts and Crosses game, we need 11 AND gates and 10 ANDAND gates.
Not tested yet, we already have planned 7AND gates. It may not be so hard to complete the set of logic gates and combine them into Noughts and Crosses.
We recently change transition diagram and found that the number of inputs can be reduced into just 6. So the implementation of SYANAC will be little bit easier.