BU06:Team A
From 2006.igem.org
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| Schedule | Research | Fundraising | Resources | Members | Contact |
|---|
| Team Goal: Photography System Assembly and Testing |
back to the BU iGEM main page
Contents |
Members
| Mac Cowell | macowell@bu.edu | aim: cxmac2 | |
| Christine Woods | cwoods@bu.edu | aim: xoptopinope | |
| Nancy Mendonca Davis | nancymd@bu.edu | ||
| Nadav Ivzan | nivzan@bu.edu | aim:kingos82 msn:kingos19@hotmail.com |
Meeting Schedule
Next meeting: Thursday, July 13th 4pm LSEB Basement
Current Progress
Photosystem parts
Light Sensing Inhibitor:
- [http://partsregistry.org/Part:BBa_I15008 BBa_I15008] (well: 21A plate: iGEM2006 DNA-2)
- [http://partsregistry.org/Part:BBa_I15009 BBa_I15009] (well: 21C plate: iGEM2006 DNA-2)
- [http://partsregistry.org/Part:BBa_I15010 BBa_I15010] (Note: Must be used in E.coli deficient in natural EnvZ.)
- [http://partsregistry.org/Part:BBa_R0082 BBa_R0082] (well: 15P plate: iGEM2006 DNA-1)
Assembly of parts
Here are instructions for transforming DNA into cells and creating our own stock of DNA from the 2006 iGEM plates as well as figuring out where your part is on the plates.
Each DNA has a certain antibiotic resistance. Plate 1 is all Ampicilin resistant. Plate 2 DNA is [http://partsregistry.org/Help:How_to_make_agar color-coded] on the bottom of each well for it's antibiotic resistance.
Questions
- What strain of e. coli should we use? How do we make those cells competent?
- How do we find out which antibiotic a particular DNA on Plate 2 is resistant to? The Biobrick Delivery instructions say that we should use the [http://partsregistry.org/cgi/assembly/libraries.cgi?id=6 Registry Repository] to find out, but it doens't seem to list each part's antibiotic resistance on there.
- The bottom of each well is [http://partsregistry.org/Help:How_to_make_agar color-coded] to indicate the antibiotic resistance.
- How do we obtain part I15010?
- Email Meagan at the registry (meaganl AT mit DOT edu) and copy James Brown (jrb62 AT cam.ac.uk)
- How do we obtain EnvZ- e. coli?
- Ask frank - he seems to know.
- Who knows what we can buy now and what general lab supplies are available for our use?
Protocols
References
- The [http://partsregistry.org/cgi/assembly/libraries.cgi index] of the 384-well plates.
- The [http://partsregistry.org/Featured_Parts:Light_Sensor Light Sensing System].
- [http://openwetware.org/wiki/Protocols Protocols]
- [http://partsregistry.org/Part:BBa_M30109 Reference Part M30109]
- Bacterial Photography [http://openwetware.org/wiki/BE.109:Systems_engineering/Basic_bacterial_photography:_black_and_white Lab] w/M30109 part.
